Society of African Journal Editors

UNIOSUN Journal of Sciences

Biodegradation of paraquat dichloride-contaminated soil with fermented corn steep

Authors: T.M. Obuotor, P.B. Yahaya, O.A. Akinloye, A.O. Sakariyau, O.A. Akinloye

Journal: UNIOSUN Journal of Sciences

Extensive use of pesticides in agriculture has led to environmental pollution and food toxicity. This study investigated the potential ability of fermented corn steep liquor in the degradation of Paraquat Dichloride (PQD) contaminated soil. One kilogram of sterile soil was spiked with three concentrations of PQD (25, 50 and 100 mgkg-1) and 100 ml fermented corn steep liquor was added. Total Heterotrophic Bacterial Count (THBC) and Total Fungal count (TFC) were determined weekly from each of the soil treatment using standard methods. Analytical Profile Index (API) kit was used for bacterial identification while fungi were identified based on microscopic characterization. The THBC ranged from 1.5 x 106 to 3.3 x 108 CFUg-1 while TFC ranged from zero to 2.2 x 107 CFUg-1. There were significant differences (p ? 0.05) in microbial count between each concentration of PQD and control. The isolated bacteria were Pseudomonas spp (54.7%), Providentia spp (7.81%), Proteus spp (7.81%), Klebsiella spp (15.6%), Citrobacter spp (12.5%) and Enterobacter spp (1.6%) while the isolated fungi were Candida spp (28.6%), Saccharomyces spp (25%), Trichophyton spp (17.9%), Penicillium spp (12.5%), Fusarium spp (10.7%) and Sporothrix spp (5.4%). Gas Chromatography-Mass Spectrometry (GC-MS) analysis revealed the percentage degradation as follows: 70% for 25 mgkg-1, 66.7% for 50 mgkg-1, 33.1% for 100 mgkg-1 while the control had 30.1%. This study revealed that fermented corn steep has the potential ability to degrade paraquat dichloridecontaminated soil.